On Tuesday June 24th, the UR SPIE student chapter will be kicking off its Annual Summer Colloquium Series. Because this is the kickoff of the colloquium series, we will be providing FREE PIZZA in addition to other snacks and refreshments!
Who: Dustin Shipp
What: Precise, time-lapsed measurements of organelle sizes in single cells by Holographic Angular Domain Elastic Scattering (HADES)
Time: 11:30 am – 12:30 pm
Where: Sloan Auditorium (Goergen 101)
Abstract: We demonstrate a label-free method of simultaneously measuring chemical and structural changes in a single cell over time. Raman spectroscopy obtains chemical information. Angular scattering allows estimates of organelle sizes. Previously, organelle size estimates in single cells were unstable due to the effects of speckle. The small illuminated field causes speckle to affect measurements of single cells more than measurements of many cells or bulk tissue. These speckle effects have been overcome through holographic angular domain elastic scattering (HADES), which records a complex scattered field that can be numerically propagated to any plane. In the image plane, we apply a series of virtual diffusers that reduce the coherence between different intracellular scatterers. Such diffusers are most easily created in silico, necessitating manipulation of the full complex field. By reducing the coherence between scatterers, HADES is able to reduce the effects of speckle and obtain stable organelle size estimates from single cells. To demonstrate the effectiveness of HADES on a biological system, we induce mitochondrial swelling and measure the organelle sizes of single cells over the next several hours. HADES and Raman spectroscopy are both well-suited to study a variety of changes in single cells over time. Prospective cellular systems for study by these methods will be discussed.
****The SPIE Student Summer Colloquium Series is a weekly event to be held on Tuesdays from June to August. If you are a student willing to give a talk, please send an email to email@example.com with your talk title, a short abstract, and preferred talk date. Do you want to know what your peers are doing in their research? Want to network with people from the same field? Would you like to benefit from scholarships and conference discounts? Well, then join SPIE now! Just go to spie.org to register to become a student member of the U of Rochester chapter or send an email to firstname.lastname@example.org if you have any questions.